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KMID : 0358419920350121768
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Korean Journal of Obstetrics and Gynecology
1992 Volume.35 No. 12 p.1768 ~ p.1778
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Effect of Gonadotropin-Releasing Hormone and Gonadal Steroids on Various Hormones Secretion in Trophoblast Cells
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지용현/Chee YH
김진우/한구택/정재근/김대훈/남궁성은/김승조/이헌영/Kim JW/Han GT/Jung JK/Kim DH/Namkoong SE/Kim SJ/Lee HY
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Abstract
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Many investigations revealed that the placenta may possess its own gonadotropin releasing hormone (GnRH) mediated human chorionic gonodtropin (hCG) and various hormones regulatory systems similar to the hyposhalamic-pituitary-gonadal axis. And the physiologic role of gonadal steroids on this hCG regulatory system has been concerned. In view of these facts, we maintained purified normal human trophoblast in a culture system, and investigated the role of GnRH and gonadal steroids in the secretion of hCG and various hormones. The effects of GnRH and gonadal steroids on the secretion of various hormones were studied using trophoblast in different gestational weeks. 1. GnRH and its analogue, Nafarelin, stimulated hCG ralease with a similar dose dependency in different gestational weeks. 2. The basal release of hCG waw lowest in the term placenta, but hCG response to GnRH was increased along with gestational weeks. 3. The levels of hCG in the GnRH increased about 10-fold of basal level 60 minutes later and reached 46-fold of basallevel 3 hours later. 4. High concentration of GnRH (10^-6 M/L to 10^-5 M/L) stimulated progesterone release with a dose dependency but did not whow any effect on estradiol secretion. 5. High concentration of GnRH (10^-6 M/L to 10^-5 M/L) stimulated LH. 6. Progesterone (0.1 micro g/ml to 10 micro g/ml) stimulated basal hCG secrection but addition of progesterone and GnRH inhibited hCG secretion in a dose dependent manner. Estradiol (0.01 micro g/ml to 10.0 micro g/ml) did not show any effect on hCG secretion. 7. The basal release of GnRH was highest at 10 weeks and was lowest in the term placenta. Secretion of progesterone was highest in the 12th week of gestation and lowest in the 8th week of gestation. It can be concluded that GnRH stimulates to release hCG and progesterone, and progesterone induces basal hCG secretion, however the addition of progesterone and GnRH inhibits hCG release in the trophoblast. These results indicate that GnRH and progesterone have an important role in the hormonal regulatory system of the placenta.
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